bio_rtd.chromatography.bt_curve

Breakthrough curves.

Breakthrough curves provide information on what ratio of inlet does not bind to the column after certain amount of material was already loaded (not all of loaded material is bound) onto the column.

btc_constant_pattern_solution

bio_rtd.chromatography.bt_curve.btc_constant_pattern_solution(m_load, dbc_100, k, cv, logger=None)[source]

Breakthrough curve - Constant Pattern Solution.

r = 1 / (1 + exp(k * (dbc_100 - m_load / cv)))

Parameters

  • m_load (ndarray) – Amount of load material already sent onto the column.

  • dbc_100 (float) – Dynamic binding capacity if the load would last indefinitely.

  • k (float) – Steepness of the breakthrough profile.

  • cv (float) – Column volume.

  • logger (Optional[RtdLogger]) – Logger for messaging about potential suspicious profiles.

Returns

r – Share of unbound material for given m_load.

r.shape == m_load.shape

Return type

ndarray